Theory
Unit I
History of plant tissue culture, principle of Totipotency; Tissue culture media; Plant hormones and morphogenesis; Direct and indirect organogenesis; Direct and indirect somatic embryogenesis; Applications of plant tissue culture; National certification and Quality management of TC plants; Genetic Fidelity testing and Virus indexing methods-PCR, ELISA [Read Here]
Unit II
Micropropagation of field and ornamental crops; Virus elimination by meristem culture, meristemtip culture and micrografting; Androgenesis and gynogenesis production of androgenic and gynogenic haploids diploidization; Protoplast culture isolation and purification; Protoplast culture; Protoplast fusion; Somatic hybridization Production of Somatic hybrids and Cybrids;, Wide hybridization embryo culture and embryo rescuetechniques; Ovule, ovary culture and endosperm culture.
Unit III
Large-scalecell suspension culture Production of alkaloids and other secondary metabolites- techniques to enhance secondary metabolite production, Somaclonal and gametoclonal variations causes and applications; Callus culture and in vitro screening for stress tolerance; Artificial seeds, In vitro germplasm storage and cryo-preservation. Commercial Tissue Culture: Case studies and success stories, Market assessment; project planning and preparation, economics, government policies