Unit I
History, scope and importance of Biotechnology; Specializations in
Agricultural biotechnology: Genomics, Genetic engineering, Tissue Culture,
Bio-fuel, Microbial Biotechnology, Food Biotechnology etc. Basics of
Biotechnology, Primary metabolic pathways, Enzymes and its activities.
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Unit II
Structure of DNA, RNA and protein, their physical and chemical properties. DNA
function: Expression, exchange of genetic material, mutation. DNA modifying
enzymes and vectors; Methods of recombinant DNA technology; Nucleic acid
hybridization; DNA/RNA libraries; Applications of gene cloning in basic and
applied research, Plant transformation: Gene transfer methods and applications
of GM crops.
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Unit III
Molecular analysis of nucleic acids -PCR and its application in agriculture
and industry, Introduction to Molecular markers: RFLP, RAPD, SSR, SNP etc, and
their applications; DNA sequencing, different methods; Introduction to genomics,
transcriptomics, ionomics, metabolomics and proteomics. Plant cell and Animal cell and tissue
culture techniques and their applications.
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Unit IV
Introduction to Emerging topics: Genome editing, gene silencing, Plant
microbial interactions, Success stories in Biotechnology, Careers and
employment in biotechnology. Public perception of biotechnology; Bio-safety
and bioethics issues; Intellectual property rights in biotechnology.
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